Comparison of periodontopathogens and detection of tetracycline resistance genes by pcr

Sri Vasavi Kadiyala and Gopinath P

Periodontal disease is the result of an imbalance in the microbial ecology of the oral cavity, and it also depends on host susceptibility.[1] Studies have shown a close association between periodontitis and the presence of a specific group of microorganisms, mainly Porphyromonasgingivalis, Aggregatibacter, Actinomycetemcomitans, Treponema denticola and Tannerella forsythia.Thus, this study is to compare the periodontopathogens and to detect the tetracycline resistance genes by PCR among patients with different periodontal conditions. In our study, T. forsythia was the most frequently detected pathogen in 5/5 (100%) chronic periodontitis and 4/5 (80%) aggressive periodontitis patients. A. actinomycetemcomitans was the least detected pathogen as it detected only one sample of chronic periodontitis case. Upon subjected them to tetracycline resistance gene (TetM gene), all were negative. T. forsythia and A. actinomycetemcomitans were the most frequently and least detected periodontic pathogens respectively. Tetracycline resistance was not observed in our isolates. This study reveals the knowledge on the prevalence of periopathogens and tetracycline resistance gene in our region.

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