Introduction: ESBL producing enterobacteriaceae have been responsible for numerous outbreaks of infection and an increase in ESBL producing enterobacteriaceae has been observed in recent years. This poses a challenging infection control issue.
Objective: Isolation and identification of ESBL producing enterobacteriaceae from clinical samples and to comparatively evaluate ESBL detection by ESBL Hichrome agar and E test. Antibiotic susceptibility testing according to CLSI guidelines.
Materials and Methods: Samples were processed using conventional methods. Bacterial etiology was identified and antibiotic susceptibility testing was done on Mueller Hinton agar according to CLSI guidelines. All enterobacteriaceae isolates were subjected to ESBL Hichrome agar plating and E test.
Results: A total of 548 enterobacteriaceae were isolated. Klebsiella species (48.1%), Escherichia coli (39.7%), Proteus species (7.6%) were the major isolates. 56.02% of all the enterobacteriaceae isolates were found to be ESBL producers by ESBL E strip method. ESBL Hichrome agar was able to detect 53.1% of enterobacteriaceae as ESBL producers.
Conclusion: It is important to identify ESBL producing enterobacteriaceae from clinical samples for the judicious use of antibiotics. For early detection of ESBL producing enterobacteriaceae isolates ESBL Hichrome agar and E tests were found to be equally effective in detecting ESBL production. ESBL Hichrome agar can be used for rapid and presumptive identification of ESBL producing enterobacteriaceae by means of growth on ESBL Hichrome agar and colony color within 24 hours with good sensitivity and specificity.
Impact Factor
2017: 6.614
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