A simple, rapid and economical method for isolating high quality DNA free from polyphenols and gum-resin is optimized for Boswellia serrata. The method significantly enhances the quality of DNA as a high purity ratio (1.73) was obtained. Minimized contamination of secondary metabolites yielded PCR amplification even after long duration storage. The method involves a modified SDS based extraction employing polyvinyl pyrrolidone (PVP) and require comparatively lesser time for DNA extraction from many samples per day than other published protocols. DNA isolated was used for random amplification of polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) assay. Amplification results of DNA ensure amenability of optimized method for molecular level studies in this species.
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