Investigation for in vitro antibacterial activity of citrus sinensis l. against propionibacterium acnes

Manisha Pandey., Afifa Qidwai., Anand Pandey., Shashi Kant Shukla., Rajesh Kumar., Ashutosh Pathak and Anupam Dikshit

Aim: The present study intends to observe and compare the bactericidal activity of the crude extract of C. sinensis L. peels in different solvent and essential oil against P. acnes and their phytochemical analysis.
Background of the Study: Acne is a chronic, inflammatory disease, of pilosebaceous unit. Hormonal imbalance, bacterial infection, stress, improper feeding habits and cosmetic applications are major factors considered to cause the development of acne. P. acnes, a gram-positive, anaerobic bacterium, has been recognized as the key factor for development of acne. Traditionally, antibiotics and hormonal doses have been applied to treat acne. However, these treatments come along with severe side effects and drug resistance.
Methodology: The phytochemical analysis of alkaloids test, flavonoids test, steroids test and saponins test were analyzed. The antibacterial activity of these extracts and essential oil were screened in accordance to globally accepted CLSI guidelines. Further, herbal extracts and oil activities were also compared with synthetic Tetracycline which was used as a standard.
Results: The results (mg/ml) indicate that ethanolic extracts of C. sinensis L. (MIC: 0.074, IC50: 0.155) as most promising form of crude extract and higher antibacterial properties as compared to petroleum ether extracts (MIC: 0.722, IC50: 0.658), peel essential oil (MIC: 1.357, IC50: 1.301) and acetone extracts (MIC: 1.390, IC50: 1.328), respectively. It therefore, provides a promising herbal supplement for the management of Acne vulgaris and that to from a waste resource.

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