Homocysteine thiolactone forms covalent adduct with arginine and histidine

Mordhwaj., Reshmee Bhattacharya., Sulekh Chandra and Vandna Singh

Available evidences suggest that homocysteine thiolactone is solely responsible for the protein N-homocysteinylation (a process in which HTL covalently modifies the targeted protein) which is responsible for the inactivation, aggregation and precipitation of proteins on target. Previous data of MS and MS/MS has already suggested the fact that HTL reacts with the side-chain ɛ-amino group of lysine residues. Spectroscopic and chromatographic analyses revealed that side-chain amino group of arginine and histidine could also prove to be potential targets for Hcy-thiolactone.

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DOI: http://dx.doi.org/10.24327/ijcar.2017.5631.0765