Effect of dietary zinc oxide nanoparticles supplementation on biochemical, hematological and genotoxicity parameters in rabbits

Hager Tarek H. Ismail and Iman E. El-Araby

Background: Nowadays, nanotechnology has opened new horizons in almost branches of veterinary sciences. The purpose of this study is to investigate the possible adverse effects of zinc oxide nanoparticles as a dietary supplement for rabbits by performing different biochemical, hematological and genotoxicity tests besides histopathological studies.

Methods: A total of sixty New Zealand White rabbits were divided randomly into four groups (Gp.1 control group, Gp. 2 zinc oxide group, Gp. 3 zinc oxide nanoparticles group and Gp. 4 zinc oxide + zinc oxide nanoparticles by half doses group). After 45 days from starting of experiment, blood and organs samples were collected and biochemical, hematological and genotoxicity testes were done beside the histopathological evaluation.

Results: Rabbits group which supplemented with zinc oxide nanoparticles showed increasing of liver enzymes activities, hypoproteinemia, hypoalbuminemia, hypoglobulinemia, reducing most of lipid profile parameters, increasing of serum creatinine and urea levels, increasing and decreasing of hepatic and renal MDA level and CAT activity respectively, no significant changes in erythrogram, leukocytosis and lymphocytosis besides genotoxic effects and histopathological changes in hepatic and renal tissues. Conclusion: Using zinc oxide supplementation in two forms (macro and nano) as a combination in rabbit’s diet reduced the adverse effects induced by ZnO NPs as whole dose and preserved the benefit of using zinc as essential element.

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