A comparative evaluation of the antibacterial efficacy of mta and biodentine against enterococcus faecalis when combined with chlorhexidine, triclosan and triple antibiotic paste

Mohsin Reza K., Krishna Prasad Shetty., Satish S V., Alexander M Luke., Ashwini M Patil and Basvana Gowda

Aim: The aim of this study was to evaluate the antibacterial efficacy of MTA and Biodentine against Enterococcus faecalis when combined with 2% Chlorhexidine, 2.5% Triclosan and 1.5% Triple antibiotic paste.
Materials & methods: The antimicrobial activities of the freshly mixed cements were evaluated by the agar diffusion method on Enterococcus faecalis.
The inoculums were prepared by making a direct brain heart infusion broth suspension of the isolated colonies from an overnight growth on blood agar plate. The suspension was adjusted to achieve a turbidity equivalent to 0.5 McFarland opacity standards. A sterile cotton swab dipped and streaked 3 times over the entire sterile agar surface, rotating the plate approximately 600 each time to ensure an even distribution of inoculums. Enterococcus faecalis were inoculated on blood agar plates.
Wells were prepared on the medium with the help of puncher of 4mm diameter and 4 mm depth and then immediately filled with freshly prepared test materials. The test materials were manipulated in accordance with the manufacturer's instructions in aseptic condition. After pre diffusion of the test materials for 2 hours at room temperature, all the plates were incubated at 370c in an incubator directly.
A blinded, independent observer then evaluated the plates at 24h, 48h, and 72h. Microbial inhibition zones were measured with a precision ruler.
Results: Efficacy between MTA and Biodentine were done by using independent "t" test for normally distributed data or Mann-Whitney test for non-normally distributed data.
A p-value less than 0.05 will be considered as significant. Antibacterial efficacy of MTA and Biodentine with experimental group showed greater inhibition zone than the control group. Group with triple antibiotic paste showed largest inhibition zone
Conclusions: Antibacterial efficacy of MTA and Biodentine enhanced with incorporation of antibacterial agents. Biodentine has greater antibacterial properties than MTA.

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