Characterization of bacteria isolated from hospital environment

Background: One of the foremost relevant public health problems is Healthcare-associated Infections (HAIs), both in high-income and developing countries.Hospital Acquired Infections or Nosocomial infections or healthcare-associated infections (HAIs) are often defined as the infections acquired within the hospital by a patient.
Objective: The objective of this study is to isolation and identification of bacterial pathogens from samples in a hospital and to evaluate their antibiotic resistance and sensitivity pattern
Method: The different samples collected were air sample, swab sample, hand swab andprocessed for culture sensitivity. The culture were subjected to different tests like Gram Stain, Catalase Test, Coagulase Test (Slide and Tube Coagulase Test), Oxidase Test, Indole Test, Citrate Test, TSI (Triple Sugar Iron) Test, Urease Test and Antimicrobial Susceptibility Test
Result: A total of 118 air samples & 179 surface swab samples were collected from different wards and OTs of the hospital, & 15 hand swab samples were collected from various personnel professionals of the hospital.Out of 118 air samples, 42.3% (50/118) were culture positive whereas 57.7 % (68/118) were culture negative. Out of 179 surface swab samples, 38.5% (69/179) were culture positive and 61.5% (110/179) were culture negative. 0ut of the 15 hand swab sample 73.3% (11/15) were culture positive and 26.7% (4/15) were culture negative.
Conclusion: The isolation of pathogenic bacteria from the hospital environment indicates that they can be vehicles for disease transmission. So, there is need for thorough disinfection and conscientious contact control procedures to minimize the spreads of these pathogens.