Thidiazuron mediated callus and multiple shoot induction in nothapodytes foetida (wight) sleumer- an important medicinal plant

Kaveri S and Srinath Rao

Mature embryo and hypocotyl explants of Nothapodytes foetida were cultured on Murashige and Skoog’s medium (MS) supplemented with different concentrations of Thidiazuron. Both multiple shoots and nodular callus from the base of shoots were induced. TDZ at 0.1 mg/l was found most effective in induction of maximum number of (14.60±0.32) shoots per explant. With an increase in the concentration of TDZ (0.2-0.4 mg/l) decrease in tendency of multiple shoot induction was noticed from both the explants. The nodular callus when subcultured again on TDZ (0.1 mg/l) supplemented media produced multiple shoots on an average of 9.25±0.29 shoots per callus. Both the explants when cultured on MS medium supplemented with 2, 4-D (2.0-4.0 mg/l) resulted in callus formation. When this callus was cultured in MS medium supplemented with TDZ (0.1-0.4 mg/l) did not responded for organogenesis.

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