Studies on isolation and characterization of some wound infection causingbacteria

Author: 
Hosimin, K and Prabakaran, G

The antimicrobial resistance pattern of aerobic bacteria isolated from burn patients admitted in plastic surgery and general surgery wards of Kumaramangalam memorial medical hospital Salem in Tamilnadu. Different types of wound samples were collected from 25 patients during the study period. Among the 25 patients, 5 types of bacterial species were isolated by selective culture medium and standard bio chemical test. Each wound samples showed one more isolates . The five isolates included Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were predominant isolates (40%) followed by Klebsiella pneumoniae and Streptococcus mutans (28%). In Escherichia coli Among the 5 different antibiotics 40% resistance showed by ciprofloxacin In Klebsiella pneumoniae Among the 5 antibiotics 28% of isolates resistance to nalidixic acid and ciprofloxacin, norfloxacin, gentamycin and tobramycin showed sensitive and intermediated to isolates. In Staphylococcus mutans , the highest resistance were showed by ampicillin (57.1%) In Pseudomonas aeruginosa , the highest resistance were showed by gentamycin (50%) In Streptococcus aureus, the highest resistance were showed by ampicillin and penicillin (90%). In Cell surface hydrophobicity, among the 39 isolates the highest activity observed from Pseudomonas aeruoginosa(98.98±0.04%). In Protease enzyme production, Totally 23% of isolates produced protease activity. In ß lactamase production, Totally 76.9% of isolates produced betalactamase activity. In Slime production, (Biofilm) all bacterial isolates produced slime activity. According to previous studies, bio film was attached to glass tube surface as positive activity. The Change in the pattern if bacterial resistance in the burn unit is important both for clinical settings and epidemiological purposes.

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