Rapid and effective method for the extraction of the genomic dna from sperm

Bhaumik D. Vaghela., Hiren S. Gajjar and Jayesh C. Jalondhara

This investigation was aimed to isolate sperm genomic DNA in quick and efficient way. The sperm samples from the authors were taken and subjected to isolation of the genomic DNA. Then isolated DNA was further subjected to amplification. Sperm DNA was isolated in nearly half an hour. The isolated DNA from both the samples was intact. Amplification of the DNA was also successful. Sperm genomic DNA can be extracted using Xcelris sperm DNA kit and can be used for various downstream applications.

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DOI: http://dx.doi.org/10.24327/ijcar.2017.3615.0331