Isolation and characterization of e. coli from urine sample and their antibiogram pattern and effect of spice extracts and natural oils against isolated e. coli

Rushita B Panvadiwala, Mayur Gahlout, Poonam B Chauhan and Hiren Prajapati

Urinary tract infections is the most common type of infection in the body. E. coli is the most frequent causative agent of UTI affecting both male and female individuals. In this study, total 151 urine samples were collected from suspected UTI patients from 2 hospitals to isolate and identify uropathogenic Escherichia coli. From this study, it was revealed that out of 151 samples, 28 samples were positive for E. coli and these showed indole and methyl red tests positive and voges-prauskar and citrate utilization tests negative. Out of twelve antibiotics, chloramphenicol, kanamycin, and ciprofloxacin were found to be the most effective antibiotics against E. coli. Among the five spice extracts, Trachyspermum ammi (ajwain), Allium sativum (garlic), and Zingiber officinale (ginger) showed maximum antimicrobial activity against E. coli. Among the six natural oils, Eucalyptus oil showed maximum antimicrobial activity against E. coli.

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