Evaluation of bacterial load in dental lamps-pilot lamp

Jayakeerthana S and Geetha

Aim: To study the bacterial load in pilot lamp.
Objective: This study focuses on finding out the bacterial load in pilot lamp in dentistry.
Background: Bacterial load is the measurable quantity of bacteria in an object,surface or medium.Apart from the habitation of bacteria in different areas of oral cavity, bacteria can also be present in dental armamentarium like dental chair, pilot lamp, dental hand piece, other storage cupboards in clinic, etc,. These bacteria don't habit these places naturally, but are mainly due to contamination. This contamination largely results from the operation of these armamentarium using gloved hands used previously for a patient. This study will thus focus on the bacterial load contamination in these armamentarium.
Method: Swabs were collected from dental clinics and were inoculated intoBrain Heart infusion (BHI) Agar using spread culture plate technique.
The culture plate was incubated for 24h at 37C and observed for microbial growth
The Colony forming Unit (CFU’s) where manually counted and tabulated as CFU/ microlitre.
Results: In this study the most predominant organisms found was micrococcus and enterococcus.

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DOI: http://dx.doi.org/10.24327/ijcar.2017.3159.0210