Development and validation of uv-spectroscopic method forestimation and validationof l-cysteine inbulk

A novel, basic and explicit UV Spectrophotometric method has been developed involving Phosphate Buffer pH 8 and Ellman’s Reagent used to estimate L-Cysteine content in bulk. The λmax of L-Cysteine was found to be 412 nm. Linearity was observed in the range of 0.25 to 1.5 mMoles having regression coefficient (R2=0.9998). The method shows linearity, accuracy, precision, LOD and LOQ as per ICH [Q2 (R1)] validation parameters and was statistically validated. Results obtained proved the applicability of current method in routine analysis of L-Cysteine in bulk.