ANTIBACTERIAL ACTIVITY OF PEPPERMINT OIL AGAINST ISOLATES OF ENTEROCOCCUS FAECALIS FROM DENTAL CARIES

The human dental cavity is colonized with large groups of aerobic and anaerobic bacterial species. Enterococcus faecalis as a nosocomial pathogen can cause serious infections that are frequently isolated (30-90%) from root canal treated patients.Peppermint oil has antibacterial activity against both gram positive and gram negative bacteria. It also possesses antiviral and antifungal activities.Thus, this study is to determine the antibacterial activity of peppermint oil against isolates of Enterococcus faecalis from dental caries populations.The MIC of peppermint oil was appeared to be 0.125% for E. faecalis peppermint oil is found to have antibacterial activity against E. facecalis. studies on toxic and irritant properties of essential oils are imperative, especially when considering any new products for human administration. -solid Antimicrobial susceptibility test Antibiotic susceptibility test was determined for these strains to routinely used antibiotics such as ampicillin (10μ), vancomycin (30μ), teicoplanin (30μ), erythromycin (15μ), ciprofloxacin (5μ), amikacin (200μ), gentamycin (10μ), tetracycline (30μ) and linezolid (30μ) (Hi Media, Mumbai) by kirby-bauer disc diffusion method. [4] Detection of antibacterial activity of peppermint oil against E. faecalis Anti-bacterial activity of peppermint oil was tested against E. faecalis isolates by minimum inhibitory concentration method.Mueller Hinton broth was supplemented with 0.002% (V/V) tween 80 (HiMedia, Mumbai) toenhance the dispersion of the essential oil. Agar dilution method was performed to attain thedifferent concentrations of essential oils such as 0.03%, 0.06%, 0.125%, 0.25%, 0.5%, 1%and 2% in Mueller Hinton Agar (MHA). Media containing various concentrations of essential oil were poured over the sterile petridishes and allowed to dry. Media without essential oil was served as control plate. Spot inoculation of 0.5 McFarland standard turbidity adjusted isolates were made on the plates and incubated at 37oC for overnight. The lowest concen completely inhibited the growth of isolates was considered as MIC. [3] 6505, Impact Factor: SJIF: 5.995


INTRODUCTION
The human dental cavity is colonized with large groups of aerobic and anaerobic bacterial species. Enterococcus faecalis as a nosocomial pathogen can cause serious infections that are frequently isolated (30-90%) from root canal treated patients. [1] The high prevalence of this species in root canal treated patients evidenced by culturing methods, and molecular detection tools suggested that it may be the reason for most of the endodontic treatment failures. [2] Peppermint oil has antibacterial activity against both gram positive and gram negative Bacteria. It also possesses antiviral and antifungal activities. The antiviral property shown to act against influenza, herpes viruses. [3]. Thus, this study is to determine the antibacterial activity of peppermint oil against isolates of Enterococcus faecalis from dental caries populations.
The human dental cavity is colonized with large groups of aerobic and anaerobic bacterial species. Enterococcus faecalis as a nosocomial pathogen can cause serious infections that are frequently isolated (30-90%) from root canal treated patients.Peppermint oil has antibacterial activity against both gram positive and gram negative bacteria. It also possesses antiviral and antifungal activities.Thus, this study is to determine the antibacterial activity of peppermint oil against isolates of Enterococcus faecalis from dental caries populations.The MIC of peppermint oil was appeared to be 0.125% for E. faecalis peppermint oil is found to have antibacterial activity against E. facecalis. studies on toxic and irritant properties of essential oils are imperative, especially when considering any new products for human administration.
The human dental cavity is colonized with large groups of aerobic and anaerobic bacterial species. Enterococcus faecalis as a nosocomial pathogen can cause serious infections that are %) from root canal treated patients. The high prevalence of this species in root canal treated patients evidenced by culturing methods, and molecular detection tools suggested that it may be the reason for most of Peppermint oil has antibacterial activity against both gram Bacteria. It also possesses antiviral and antifungal activities. The antiviral property shown to act against influenza, herpes viruses. [3]. Thus, this ermine the antibacterial activity of peppermint oil against isolates of Enterococcus faecalis from dental caries repetitive dental caries isolates of collected included in this study. These isolates were identified by standard biochemical parameters as described by elsewhere. Isolates were preserved in semi-solid brain heart infusion medium and stored at 4ºC until further

Detection of antibacterial activity of peppermint oil against E. faecalis
Anti-bacterial activity of peppermint oil was tested against E. faecalis isolates by minimum inhibitory concentration method.Mueller Hinton broth was supplemented with 0.002% (V/V) tween 80 (HiMedia, Mumbai) toenhance the dispersion of the essential oil. Agar dilution method was performed to attain thedifferent concentrations of essential oils such as 0.03%, 0.06%, 0.125%, 0.25%, 0.5%, 1%and 2% in Mueller Hinton Agar (MHA).
Media containing various concentrations of essential oil were poured over the sterile petridishes and allowed to dry. Media without essential oil was served as control plate. Spot inoculation of 0.5 McFarland standard turbidity adjusted isolates were made on the plates and incubated at 37ºC for overnight. The lowest concen completely inhibited the growth of isolates was considered as MIC. [3]

ANTIBACTERIAL ACTIVITY OF PEPPERMINT OIL AGAINST ISOLATES OF ENTEROCOCCUS aveetha Dental College, Chennai
The human dental cavity is colonized with large groups of aerobic and anaerobic bacterial nosocomial pathogen can cause serious infections that 90%) from root canal treated patients.Peppermint oil has antibacterial activity against both gram positive and gram negative bacteria. It also gal activities.Thus, this study is to determine the antibacterial activity of peppermint oil against isolates of Enterococcus faecalis from dental caries populations.The MIC of peppermint oil was appeared to be 0.125% for E. faecalis.The found to have antibacterial activity against E. facecalis. However, the studies on toxic and irritant properties of essential oils are imperative, especially when considering any new products for human administration.

Antimicrobial susceptibility test
Antibiotic susceptibility test was determined for these strains to routinely used antibiotics such as ampicillin (10µ), teicoplanin (30µ), erythromycin (15µ), ciprofloxacin (5µ), amikacin (200µ), gentamycin (10µ), tetracycline (30µ) and linezolid (30µ) (Hi Media, Mumbai) by bauer disc diffusion method. [4] Detection of antibacterial activity of peppermint oil against bacterial activity of peppermint oil was tested against E. faecalis isolates by minimum inhibitory concentration method.Mueller Hinton broth was supplemented with 0.002% (V/V) tween 80 (HiMedia, Mumbai) toenhance the dispersion sential oil. Agar dilution method was performed to attain thedifferent concentrations of essential oils such as 0.03%, 0.06%, 0.125%, 0.25%, 0.5%, 1%and 2% in Mueller Media containing various concentrations of essential oil were over the sterile petridishes and allowed to dry. Media without essential oil was served as control plate. Spot inoculation of 0.5 McFarland standard turbidity adjusted isolates were made on the plates and incubated at 37ºC for overnight. The lowest concentration of the essential oils that completely inhibited the growth of isolates was considered as

Research Article
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Antibiotic susceptibility pattern
We found increased percentage of isolates were shown to be resistant to all the antibiotics used in this study. For ampicillin, amikacin, erythromycin, gentamicin, our isolates were found to resistant between 80-90%. Better sensitivity was observed in linezolid, teicoplanin and vancomycin antibiotics. The detailed results of antibiotic sensitivity patter of Enterococci was given in table 1.

Antibacterial activity of peppermint oil against isolates of Enterococcus faecalis
We have observed that, E. faecalis from dental carieswere inhibited from 0.125-1% of peppermint oil.
The MIC of peppermint oil was appeared to be 0.125% for E. faecalis.

CONCLUSION
The peppermint oil is found to have antibacterial activity against E. facecalis. However, the studies on toxic and irritant properties of essential oils are imperative, especially when considering any new products for human administration. This can be used as alternative and complementary antibacterial agents for controlling dental caries. *******